“Zero-Crossing” First-order Derivative Spectroscopy Method for Analysis of Sertraline Hydrochloride in Novel Self Micro Emulsifying Drug Delivery System

Research Article

Austin J Anal Pharm Chem. 2014;1(3): 1013.

“Zero-Crossing” First-order Derivative Spectroscopy Method for Analysis of Sertraline Hydrochloride in Novel Self Micro Emulsifying Drug Delivery System

Khutle Nilesh M1, Pawar Harshal A2* and Vijaya C3

1Department of Pharmaceutics, Dr. L. H. Hiranandani College of Pharmacy, I

2Dr. L. H. Hiranandani College of Pharmacy, India

3Department of Pharmaceutics, Ultra College of Pharmacy, India

*Corresponding author: :Pawar Harshal A, Department of Pharmaceutics, Dr. L. H. Hiranandani College of Pharmacy, Madurai, Tamilnadu, India.

Received: August 26, 2014; Accepted: September 10, 2014; Published: September 11, 2014

Abstract

Novel Self-Micro Emulsifying Drug Delivery System (SMEDDS) of Sertraline hydrochloride was developed. SMEDD System of Sertraline hydrochloride consists of Capmul MCM C8 EP as oily phase, Cremophore RH 40 as surfactant and Labrafil M 2125 as co surfactant. These self-emulsifying additives interfere significantly with the estimation of Sertraline hydrochloride by UV spectrophotometry method. In the present study “Zero crossing” first-order derivative spectroscopy method was developed and validated to estimate the Sertraline hydrochloride in presence of self emulsifying additives and compared with simple UV-Visible spectrophotometry method. The obtained results noticeably recommended that, in comparison with the UV-spectrophotometry method; the first-order derivative UV-spectrophotometry is a simple method to estimate Sertraline hydrochloride with sufficient accuracy, specificity and precision. The proposed methods were applied successfully to estimate the drug content and in vitro drug release of newly formulated Self emulsifying drug delivery system of Sertraline hydrochloride.

Keywords: Sertraline hydrochloride; Self Emulsifying Drug Delivery; First order derivative spectrophotometry

Abbreviations

SRT: Sertraline Hydrochloride; SMEDDS: Self-microemulsifying Drug Delivery System; RSD: Relative Standard Deviation; LOD: Limit of Detection; LOQ: Limit of Quantitation; SAB: 0.05 M Sodium Acetate Buffer, pH 4.5; SGF: Simulated Gastric Fluid; 0.1 N HCl; SIF: Simulated Intestinal Fluid; Phosphate Buffer pH 6.8

Introduction

Sertraline hydrochloride; (1-S, cis)-4-(3, 4-dichloroph-enyl)- 1,2,3,4-tetrahydro-N-methyl-1-naphthalene amine (Figure 1), is a antidepressant of the selective serotonin reuptake inhibitor (SSRI). Sertraline hydrochloride has become one of the most commonly used medications for the treatment of depression, generalized anxiety disorder, social anxiety disorder, panic disorder, obsessive compulsive disorder and posttraumatic stress disorder [1].