Compositional Study and Antioxidant Potential of Polyphenols Extracted from Corn By-Products, Using Ultrasound Extraction Method

Research Article

Austin Chromatogr. 2016; 3(1): 1043.

Compositional Study and Antioxidant Potential of Polyphenols Extracted from Corn By-Products, Using Ultrasound Extraction Method

Aires A¹* and Carvalho R²

¹Centre for the Research and Technology for Agro- Environment and Biological Sciences (CITAB), Universidade de Trás-os-Montes e Alto Douro, UTAD, Vila Real, Portugal

²Agronomy Department, Universidade de Trás-os-Montes e Alto Douro, UTAD, Vila Real, Portugal

*Corresponding author:Aires A, Centre for the Research and Technology for Agro-Environment and Biological Sciences, CITAB, Universidade de Trás-os- Montes e Alto Douro, UTAD, Quinta de Prados, Vila Real, Portugal

Received: May 10, 2016; Accepted: July 11, 2016; Published: July 18, 2016

Abstract

The aim of this research was to determine the polyphenol composition and antioxidant activity of corn silk hairs. Two different methods were used to extract polyphenols: a (i) conventional extraction method with 70oC and 15 min; and an (ii) ultrasound extraction method with 40 kHz, for 15 minutes. A HPLCDAD- UV/VIS system as was used to detect the polyphenols in the samples. The total antioxidant activity was measured by the spectrophotometric ABTS●+ free radical assay. The main polyphenols found were caffeic acid, chlorogenic acid, ferulic acid, pelargonidin and apigenin. The average content of each polyphenol detected as well as the total antioxidant activity were significantly dependent (0.05Z&P<0.001) of extraction method used and both were higher in samples extracted under ultrasounds. The total antioxidant activity was highly correlated with the presence of pelargonidin (P<0.01), ferulic acid (P<0.01) and apigenin (P<0.01). Based in our results, corn silk hairs can be used to extract polyphenols with enhanced biological activity and the ultrasounds shown to be very practical, feasible and accurate to maximize their polyphenol content. The knowledge regarding the composition of corn silk hairs can enable their use in pharmaceutical or industrial heath sector to produce coproducts with high biological added value.

Keywords: Corn wastes; Phytochemicals; Bioactivities; Enhanced value

Introduction

Corn (Zea mays L.) silk hairs also known as Maydis stigma is a popular corn byproduct which has been used for decades as medicinal herb in folk medicine of different countries all over the world even if the scientific evidences of their beneficial effects on human health are often contradictory. Corn silk hairs are usually considered as waste and they are discarded during industrial transformation of baby corn and super sweet corn products. They contain several nutritional compounds such as proteins, vitamins and minerals as well as phytochemical like phenolic acids, flavonoids and other polyphenols, which are claimed to be responsible for several beneficial effects on human health [1]. Corn silk hairs has been claimed to be a good herb to treat depression, hypertension, hyperglycemia, hepatitis, cystitis, gout, kidney stones, diabetes, nephritis and prostatitis [2-6]. Other uses include teas, powders and supplements to treat urinary related problems [7], which have been endorsed to the higher content in flavonoids [8]. Although the derived-products from corn silks such as pills, powders, teas or even other formulations, have been highly marketable all over the world, corn silk hairs are still considered as a waste and highly disregarded by corn industrial producers. As consequence, the opportunities to convert them into high added value coproducts are still limited. Thus, with this study we aim to reinforce the scientific knowledge about the beneficial effects of corn silk hairs trough the evaluation of their polyphenol composition and antioxidant capacity and how those properties linked each other and are affected with extraction method used. Also we want to evaluate if the ultrasonic treatment can be furthermore industrially developed for nutraceuticals and functional coproducts applications.

Material and Methods

Sampling

1 kg of corn silk hairs (Zea mays subsp. Mays L.) were collected, dried in a freeze-drier system (UltraDrySystemsTM, USA), milled into a fine powder, and stored in dark at 4°C until the extraction procedure.

Extraction procedure

Two different extractions methods, conventional and Ultrasound (UE) were performed in order to compare and establish a simple, practical and feasible extraction procedure. In the conventional extraction 100 mg of dried powered was placed in a 10 mL screw cap tubes and mixed with 10 mL of extraction solvent (70% methanol), followed by a vigorous agitation in a vortex (Genie 2, Fisher Scientific, UK). The tubes were then sealed and heated at 70°C (1083, GFL-Gesells chaft ffur Labortechnik mbH, Germany) during 15 minutes, and agitated every 5 minutes. The obtained extracts were then centrifuged (4000 rpm for 15 min (Kubota, Japan)), filtered consecutively through a Whatman No. 1 paper and syringe filters (0.2μm, 13mm Ø, Teknokroma, Spain) to a HPLC amber vials. The extracts were stored at -20°C prior to the LC analysis.

In the UE, the mixtures of 100 mg of dried powder with 10 mL of 70% methanol were placed in an ultrasonic bath device (34cm×10cm×31cm) with 40 kHz transducers annealed to a rectangular container (Bandelin Electronics, Berlin, Germany).The tubes were then sealed and irradiated for the pre-set extraction time (15 minutes). Then, the extracts were centrifuged (4000rpm for 15 min.) and the supernatants filtered, first in a Whatman No.1 paper Spartan filters. Finally, the extracts were filtered (0.2μm, 13mm Ø, Teknokroma, Spain) to amber flasks until HPLC analysis.

Extract purification and HCL hydrolysis

To remove impurities and undesirable non-phenolic compounds from the samples, a purification trough resin Sephadex LH20 (Sigma- Aldrich, Tauferkichen, Germany) and an HCl (Sigma-Aldrich, Tauferkichen, Germany) hydrolysis were performed. The columns with Sephadex LH-20, were firstly washed with 10 mL of ultrapure water followed by activation with 30 mL of methanol 20% (methanol: water). Then, 10 mL of extract were applied in the columns, followed by washing with 10mL of methanol 20%. After, the compounds were eluted with 10mL methanol 60%. An aliquot of 300μL of each eluted sample was evaporated until complete dryness. Then the residues were ressuspended in same volume of HCl (2 M in methanol 50%) and heated at 80°C during 120 minutes. After the extracts were filtered through a syringe filters to HPLC vials amber and stored at -20°C, until further analysis in HPLC.

Compositional determination by HPLC-DAD-UV/Vis

The phytochemical composition was assessed by HPLC-DADUV/ Vis system [9]. The eluent was constituted by water with 1% of Trifluoroacetic Acid (TFA) (solvent A) and acetonitrile with 1% TFA (solvent B). Elution was performed using a flow rate of 1 mL.min-1 of solvent and a gradient starting with 100% of solvent A. The injection volume of 10μL. Chromatograms were recorded at 280, 320, 370 and 520nm with a C18 column (250 x 46mm, 5μm). The phytochemicals were identified using peak retention time, UV spectra and UV max absorbance bands, compared with those found for external commercial standards. The external standards apigenin, cafeic acid, chlorogenic acid, ferulic acid AND pelargonidin were purchased from Extrasynthese (Cedex, France). All standards were freshly prepared in 70% methanol (methanol: water) at 1.0mg.mL- 1. The quantification was performed using the internal standard (naringin, Sigma-aldrich, Germany) method and the levels were expressed as μg.g-1 dry weight (dw). Methanol and acetonitrile were purchased from Panreac chemistry (Lisbon, Portugal) and Sigma- Aldrich (Taufkirchen, Germany), respectively. The aqueous solutions were prepared using ultra-pure water (Milli-Q, Millipore).

Total Antioxidant Activity (TAA)

The TAA was measure trough the in vitro assay of 2,2'-azinobis- 3-ethylbenzothiazoline-6-sulphonate (ABTS●+) radical cation [10] in a 96-well microplate. ABTS was generated by mixing a 7mM of ABTS at pH 7.4 (5mM NaH2PO4, 5mM Na2HPO4 and 154mM NaCl) with 2.5mM potassium persulfate (final concentration) followed by storage in the dark at room temperature for 16 h before use. The mixture was diluted with ethanol to give an absorbance of 0.70 ± 0.02 units at 734 nm using spectrophotometer (U-2000, serial 121-0120, Hitachi Ltd., Tokyo, Japan). For each sample, diluted methanol solution of the sample (100μL) was allowed to react with fresh ABTS solution (900μL), and then the absorbance was measured 6 min after initial mixing. The TAA was expressed as follows: TAA (%) = [((solvent absorbance - sample absorbance) / solvent absorbance) ×100]. All determinations in were performed in triplicate.

Statistical analysis

The results were expressed as mean values ± Standard Deviation (SD) of three replications. A two-way statistical analysis (ANOVA) was performed and Pearson’s correlation coefficients were calculated.

The software SPSS v.17 (SPSS-IBM, Orchard Road-Armonk, New York, USA) was used to carry out these analyses.

Results and Discussion

Method validation and analytical quality assurance

The method was validated for quantitative measurements using calibration curves and the analytical parameters tested were selectivity, linearity, precision, accuracy and recovery, Limit of Detection (LOD) and Limit of Quantification (LOQ). Five calibration curves for chlorogenic acid, caffeic acid, ferulic acid, apigenin and pelargonidin with 5 points each were established in the concentration range of 5.0 to 100 to μg.mL-1. The LODs and LOQs were evaluated from the slope and residual standard deviation of the respective standard curves. An accuracy by spiking recovery test was assayed in which we prepared and injected in HPLC an amount of each polyphenol, ranging from 50 to 100 μg.mL-1, followed by the respective determination of the amount found. The reference spiked samples were treated and analyzed using the same procedure adopted for the plant samples. Recovery rate was calculated by comparing the amount of each polyphenol detected in the spiked sample with the amount of each standard added. Instrumental precision was determined by replicate analysis of standard compounds (n=5). SPSS for windows version 17.0 were used to calculate all statistical parameters (means, standard deviations, coefficient of variation, minimum and maximum, correlation coefficient), and a t-test was used for determination of significant differences between the mean values. The method performance data are shown in Tables 1 and 2.

According our results, the extraction and quantification method demonstrated a good linearity, with R2>0.99 Table 1, and recovered of polyphenols varied between 92.6 and 120.7% Table 2. The method showed good selectivity, since all polyphenols detected were well separated from other compounds with good resolution.