The pLoc_bal-mGneg Predictor is a Powerful Web-Server for Identifying the Subcellular Localization of Gram- Negative Bacterial Proteins based on their Sequences Information Alone

    

    

    Figure 2: A semi screenshot for the webpage obtained by following Step 3 of
Section 3.5 (Adapted from [4] with permission).
    

In addition to the advantages of high accuracy and easy to use, the web-server has been constructed by strictly observing the “Chou’s 5-steps rule” and hence bears the following fantastic merits as agreed and supported by many investigators (see, e.g., [10-91] as well as three comprehensive review papers [2,92,93]): (1) crystal clear in logic development, (2) completely transparent in operation, (3) easily to repeat the reported results by other investigators, (4) with high potential in stimulating other sequence-analyzing methods, and (5) very convenient to be used by the majority of experimental scientists.

Besides, the approach [94-96] of PseAAC (Pseudo Amino Acid Composition) has also been applied during developing the webserver predictor. It is a very powerful approach for representing the protein samples by catching their special or key features, as done by many scientists as well [97-222].

Furthermore, the IHTS (Inserting Hypothetical Training Samples) treatment has also been applied to balance or quasi-balance out the training dataset [57,60,84].

For the amazing and awesome roles of the “5-steps rule” and general “PseAAC” in driving proteome, genome analyses and drug development, see a series of recent papers [2,93,223-232] where both the “5-steps rule” and the general “PseAAC” as well as their huge impacts have been very impressively recollected at different angles or from varieties of aspects.

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