New Multi Wavelength Method for the Estimation of Tazarotene and Hydroquinone in Gel Formulation

Research Article

Austin J Anal Pharm Chem. 2018; 5(2): 1099.

New Multi Wavelength Method for the Estimation of Tazarotene and Hydroquinone in Gel Formulation

Rakesh Yadav*, Rajkumar Yadav and Divya Yadav

¹Department of Pharmacy, Banasthali University, Banasthali-304022, Rajasthan, India

*Corresponding author: Rakesh Yadav, Department of Pharmacy, Banasthali University, Banasthali-304022, Tonk, Rajasthan, India

Received: April 20, 2018; Accepted: May 25, 2018; Published: June 01, 2018


A simple, sensitive, rapid, precise & accurate, UV spectrophotometric method has been developed for simultaneous estimation of Tazarotene & Hydroquinone from their gel formulation. This method is based on multi wavelength spectroscopic method. For the simultaneous estimation of both the drug sampling wavelength 294nm and 351nm were selected. Tazarotene and Hydroquinone showed linearity in concentration range of 1-5 μg/ml and 10-50 μg/ml respectively. Recovery for tazarotene and hydroquinone was obtained in the range of 99.38% to 100.0%. All three methods showed good reproducibility and recovery with % RSD less than 2.0%. Statistical validation of data shows that the proposed methods can be successfully applied for routine analysis of drugs in gel formulation.

Keywords: Tazarotene; Hydroquinone; Simultaneous estimation; Multicomponent


Hydroquinone is benzene-1, 4-diol chemically or also known as quinol having the chemical formula C6H4(OH)2 [1]. It is topical agent used for treatment of certain skin conditions. In skin creams it is also used as de-pigmenter agent and antioxidant in the photography industry. Hydroquinone acts by inhibiting the melanin formation. Due to toxicological effects of hydroquinone it can cause dermatitis. In skin toning creams hydroquinone and some of its derivatives are present. So the determination of hydroquinone and its derivative in cosmetics is very important for the protection of human health [2-4].

Tazarotene is member of the acetylenic class of retinoids. Tazarotene is chemically (ethyl 6-[2-(4, 4-dimethyl-3, 4-dihydro- 2H-1-benzothiopyran-6-yl)-ethynyl]-pyridine-3-carboxylate). It is a third-generation topical retinoid. It is available in the form of cream, gel, or foam. Tazarotene is used for treatment of psoriasis, acne and photo damage skin.

Tazarotene is a prodrug which is converted to its active form by rapid de-esterification in humans and animals. Tazarotenic acid binds to all three members of the retinoic acid receptor (RAR) family: RARa, RARβ, and RARγ but shows relative selectivity for RARβ, and RARγ which may modify gene expression. The clinical significance of these findings is unknown [5] (Figure 1).