Presence of Vaginal Secretion on Accused’s Underwear in Absence of Semen and Blood on Victims Swabs/Clothings as a Proof of Sexual Assault: A Report

Case Report

Austin J Clin Case Rep. 2019; 6(3): 1147.

Presence of Vaginal Secretion on Accused’s Underwear in Absence of Semen and Blood on Victims Swabs/Clothings as a Proof of Sexual Assault: A Report

Chakravarty M1,2*, Singh N1, Sharma D1, Sharma N1, Pandya P2 and Raina A3

¹Forensic Science Laboratory, Home Department, Government of NCT Delhi, Rohini, India

²Amity Institute of Forensic Sciences, Amity University, Noida, Uttar Pradesh, India

³All India Institute of Medical Sciences, Delhi, India

*Corresponding author: Chakravarty M, Forensic Science Laboratory, Home Department, Government of NCT Delhi, Sector14, Rohini, Delhi, India

Received: April 29, 2019; Accepted: June 03, 2019; Published: June 10, 2019


The sexual assault cases are on a rise now a days. Here we present a case of an eleven years old child who was a victim of sexual abuse. On preliminary examination, blood and semen could not be detected on the swabs/clothing’s of victim. However, some white stains were visible on the underwear of the accused, which tested negative for semen. DNA profiling was conducted for the samples. Multiplex STR typing was used to study the 15 STR Loci in addition to a gender identification marker, amelogenin by capillary electrophoresis on 3500 xl Genetic Analyzer. It was observed that the profiles generated from the underwear of accused matched with the victim’s DNA profile. Thus with DNA fingerprinting technique the case was concluded, linking the victim and the assailant even in the absence of semen and blood on the samples of victim.

Keywords: Semen; DNA; Loci; Electrophoresis; STR


Human genome comprise of 3x109 base pairs. Out of these only 30,000 base pairs are coding & the rest non coding DNA sequences. STR loci are the polymorphic regions found in the genome that are used as genetic markers for human identification-forensic case work, data basing and paternity testing. DNA fingerprinting was first described by Jeffrey et al. [1]. They found that certain regions of DNA contain repeated DNA sequences which they referred to as variable number of tandem repeats. The microsatellites are short sequences of nucleotides; 3-7 base pairs repeated over and over again a variable number of times in tandem. Polymorphic STR loci are the most informative PCR based genetic markers for attempting to individualise biological material due to variation between individual found in these regions. The combination of DNA based tests and regularly used cytological tests have increased male evidence detection rates in forensic case work [2].

Case Presentation

Fifty three percent of children in India face some from of child sexual abuse [3]. With childhood sexual abuse, victims are after too young to know how to express or to know what is happening. When not treated properly it leads to depression & anxiety. Here we report a case, where a 10yrs. old child was undertaking music classes from a tutor aged 37yrs. since last 2 years. One evening the mother of the victim saw the daughter lying in bed without clothes and this tutor leaving the room silently. The child narrated to her mother that the tutor strips her clothes daily and assaults her. FIR was lodged on the very next day of the incident under Protection of children against sexual offence Act, 2012 [4] & 376 IPC POCSO. POCSO Act was passed by both the houses of Parliament on May 22, 2012. The act defines a child as any person below the age of 18. It deems a sexual assault to be ‘aggravated’ under certain circumstances, such as when the abused child is mentally unstable or when the abuse is committed by a person in a position of trust or a authority like a family member, family friend, teacher or doctor. Punishments include imprisonment from not less than at least 3 years extending up to 7 years to life imprisonment and a fine. According to IPC section 376, “whoever, except in the case provided for in sub section commits rape, shall be punished with rigorous imprisonment of either description for a term which shall not be less than seven years but which may extend to imprisonment for life, and shall also be liable to fine.”