Extraction, Physicochemical Characterization and Pharmacological Evaluation of Alginate from a Mediterranean Species of Brown Algae Dictyopteris membranaceae

Research Article

J Drug Discov Develop and Deliv. 2022; 8(1): 1039.

Extraction, Physicochemical Characterization and Pharmacological Evaluation of Alginate from a Mediterranean Species of Brown Algae Dictyopteris membranaceae

Ayadi R¹*, Msahli A², Hamdi A¹, Majdoub H² and Bouraoui A¹

1Department of Drug Development, Faculty of pharmacy, University of Monastir, Tunisia

2Department of Medicinal Chemistry Laboratory, Faculty of Sciences, University of Monastir, Tunisia

*Corresponding author: Rihab A, Department of Drug Development, University of Monastir, Monastir, 5000, Tunisia

Received: March 02, 2022;Accepted: March 24, 2022; Published: March 31, 2022


Brown seaweeds have attracted the attention of research. Alginates, one of the components of brown seaweed, are the subject of our study. In this work, we present the extraction, the physico-chemical characterization and the pharmacological evaluation of alginate from the brown seaweed: Dictyopteris membranaceae. The extraction of alginates from brown seaweed was conducted using Rioux et al. method with few modifications. The characterization of alginates was then preceded by infra-red spectroscopy. The content of different components was determined using Ultraviolet-visible spectroscopy. Molecular properties were determined using size exclusion chromatography. The results of our study showed that the yield of extraction of alginate from Dictyopteris membranacea is 7% of dry weight. Protein, total sugar and alginic acid content were determined using colorimetric assays. The results are comparable to those reported in literature. The infra-red spectrum confirmed the nature of our extract. The molecular weight is 77823 Daltons. Additionally, our extract presented a higher gastroprotective activity at 200mg/Kg than sucralfate administered at the same dose. Our results suggest that alginate from Dictyopteris membranaceae could be used as a gastroprotective agent.

Keywords: Sodium alginates, Dictyopteris membranaceae


The marine world is of enormous scientific interest as it houses living organisms with unique structures [1,2], metabolic pathways, reproductive systems, sensory mechanisms and defenses due to their adaptation to unprecedented extreme environmental conditions [3]. Mediterranean coral reefs bring together a heterogeneous set of marine organisms. Among which we find algae. These are made up of a large group of marine plants of spectacular biological importance allowing them to be an attraction for exploitation in research and development. It has also turned out that algae have a ubiquitous presence. They are found on rocks, dead corals and stones [4]. The subject of our study is Dictyopteris membranaceae, brown seaweed up to 10cm in height with a flattened, slender yellowish-brown thallus. Dictyopteris membranaceae contains a considerable amount of content, reports have shown variation in percentages which is linked to the period of harvest and also the geographic location of the species. Alginate is one of the most popular polysaccharides in Dictyopteris membranaceae which can come up to 18.93% of dry weight [5]. Alginate is a negatively charged random or alternating linear copolymer [6]. It is composed of residues of (1→4) α-L-glucuronic (G) and β-D-mannuronic (M) (Figure 1). The proportion and their linear arrangement varies from one species of algae to another, from the part of the algae, and even from the time of harvest [7,8].