Cardiovascular Diseases Associated Inflammatory Biomarker Levels in a Small Cohort of HIV-1 Infected Patients Deintensificated from Abacavir/Lamivudine/ Dolutegravir to Lamivudine Plus Dolutegravir Running Head: Risk of Cardiovascular Diseases and Abacavir Use

Research Article

Austin J HIV/AIDS Res. 2021; 7(1): 1049.

Cardiovascular Diseases Associated Inflammatory Biomarker Levels in a Small Cohort of HIV-1 Infected Patients Deintensificated from Abacavir/Lamivudine/ Dolutegravir to Lamivudine Plus Dolutegravir Running Head: Risk of Cardiovascular Diseases and Abacavir Use

Be G, Lattuada E, Gibellini D, Diani E, Coledan I, Luise D, Tacconelli E and Lanzafame M*

Department of Diagnostic and Public Health, University of Verona, Unità Complessa di Malattie Infettive, Italy

*Corresponding author: Massimiliano Lanzafame, Department of Diagnostic and Public Health, University of Verona, Unità Semplice organizzativa di “Diagnosi e terapia dell’Infezione da HIV”, Unità Complessa di Malattie Infettive, via Piazzale L Scuro 10, Verona, Italy

Received: April 14, 2021; Accepted: May 13, 2021; Published: May 20, 2021

Introduction

Following the successful introduction of combined Antiretroviral Therapy (cART), a dramatic decrease in viral burden and opportunistic infections along with a consistent increase in life expectancy has been observed in Human Immunodeficiency Virus (HIV) infected patients [1]. This deep change in the HIV disease evolution has determined that HIV positive subjects were effectively monitored for several alterations of many tissue and organs due to HIV chronic disease and antiretroviral treatment for example, cardiovascular system, bone, adipose tissues, kidney and central nervous system represent the major target of these structural and functional damages during HIV infection.

In particular, Cardiovascular Diseases (CVD) were considered important clinical complications in the HIV patient and represent a leading cause of death among HIV-positive patients, accounting for approximately 11% of the total deaths in this population [2]; the risk of CVD is higher in HIV positive individuals compared with HIV negative people, and particularly the reported Myocardial Infarction (MI) incidence in cohort study ranges from 3 to 11 cases per 1000 patients a year in HIV- positive individuals against 2 to 7 cases per 1000 patients-years in HIV-negative population [3,4].

Although initial studies indicated a higher prevalence of traditional CVD risk factors in HIV infected population [5,6] as a possible cause, the molecular mechanisms of increased CVD risk in HIV still remain incompletely defined and should be probably attributable to a combination of multiple factors, including both direct and indirect effects of HIV infection on metabolism. Evidence from experimental and observational studies [7,8] in recent years suggested a more important role of HIV itself in contributing to CVD.

Endothelial dysfunction due to gp120, Tat and Nef proteins have been identified as a critical link between infection, inflammation, immune activation, atherosclerosis and cardiovascular system. Moreover, ART may play a role in the exacerbation of risk factors for CVD [9]; since the presentation of findings from the Data Collection on Adverse Events of Anti-HIV Drugs (D:A:D) study in 2008 demonstrating a 90% increased risk of MI in HIV- positive individuals receiving ART regimens including Abacavir (ABC), subsequent studies, conducted by FDA [10], GlaxoSmithKline [11] and independent researchers [12], to investigate this risk have yielded conflicting results.

Although more recent studies have shown an effective increased risk of CVD associated with use of ABC, many results did not reach statistical significance [13-17]. The absence of a demonstrated underlying biological mechanism for such a risk added interest and confusion about the question, as well as the higher prevalence of risk factors for CVD, such as renal impairment and substance abuse among abacavir recipients; in addition, a recent meta analysis suggests that Relative Risk (RR) for MI is increased within a 6 months exposure to ABC (RR=1.61; 95% confidence interval: 1.48–1.75) and in cART-naive population [18].

While the published evidence remains conflicting and a plausible biological mechanism for this potential association has not yet been identified, in the following study we have tried to verify whether, after introduction of ABC and its discontinuation in the contest of HAART deintensification, common metabolic markers CVD related such as glucose, LDL, HDL, total cholesterol and triglycerides and inflammatory biomarkers such as IL-6 and D-dimer could change in a small cohort of HIV-1 infected patients.

Materials and Methods

As described in a previous article [21], from March to December 2016 we enrolled 20 HIV-1 infected patients. 17 patients were still in follow-up (12 men and 5 women) in August 2020, and all were taking lamivudine plus dolutegravir. HIV-1 infected patients were not coinfected with hepatitis B and/or hepatitis C viruses. All the enrolled subjects gave informed consent to all procedures in accordance with the Helsinki Declaration.

Blood samples were collected at HIV-1 infection diagnosis before starting a three-drugs regimen consisting in ABC/3TC/ DTG (T0) , at 12 months (T2) and 12 months (T3) after therapy deintensification to lamivudine plus DTG (DITT; deintensification treatment time) [22].

Inclusion criteria included: I) naive to ART patients affected by HIV chronic infection, II) undetectable HIV-RNA levels (<50 copies/ ml) for over 12 months with a three-drugs ART treatment consisting in ABC/3TC/DGT at T2, III) undetectable HIV-RNA levels (<50 copies/ml) for over 12 months with a dual ART regimen consisting in lamivudine plus DGT at T3, iv) absence of adverse drug reactions with the proposed regimens, v) no use of statins and lipid-lowering therapy at the enrolling time and at DITT.

Blood samples were collected by venipuncture during the follow up, 30 days before the DITT (T2) and 12 months after the DITT (T3). Every blood sample collected consisted in quantitative plasmatic determination of glucose, IL6, D-Dimer and plasmatic lipids such as triglycerides, LDL, HDL and total cholesterol.

Biomarkers plasmatic levels were evaluated with specific commercial kits.For IL-6 we used Human IL-6 high sensitivity ELISA kit (Diaclone,Besancon,France);for D-dimer RayBio Human D-dimer ELISA Kit (RayBiotech, Norcross, GA, USA). Values of different samples were evaluated between the different time groups (T1, T2 and T3 groups) with statistical non-parametric procedures such as Wilcoxon test and Friedmann test using GraphPad Prism 7 software. Data were considered significant with p value <0.05 [23,24].

Results

We analyzed a cohort of 17 HIV-1 infected patients (12 men and 5 women, aged from 24 to 70 years, average 41.35 years old) infected by HIV-1 B subtypes. All mean and median values for each group are reported in Table 1, together with normal reference values. Cytokine IL-6 did not show any significant amount of variation among the three groups of samples (Group 1 vs 2 p=0.83; Group 1 vs 3 p=0.65; Group 2 vs 3 p= 0.97 Wilcoxon test). The statistical analysis of the three groups confirmed these data (p=0.645; Friedmann test). Similarly, D-dimer did not significantly change its levels in serum (Group 1 vs 2 p=0.32; Group 1 vs 3 p=0.38; Group 2 vs 3 p= 0.69 Wilcoxon test; p=0.53 Friedmann test).

Citation: Be G, Lattuada E, Gibellini D, Diani E, Coledan I, Luise D, et al. Cardiovascular Diseases Associated Inflammatory Biomarker Levels in a Small Cohort of HIV-1 Infected Patients Deintensificated from Abacavir/ Lamivudine/Dolutegravir to Lamivudine Plus Dolutegravir Running Head: Risk of Cardiovascular Diseases and Abacavir Use. Austin J HIV/AIDS Res. 2021; 7(1): 1049.