Innovative Wound Dressing Coated with Drug-Loaded Adipose Mesenchymal Stem Cells to Promote Wound Healing in Diabetes

Research Article

Austin J Med Oncol. 2023; 10(1): 1075.

Innovative Wound Dressing Coated with Drug-Loaded Adipose Mesenchymal Stem Cells to Promote Wound Healing in Diabetes

Yinglin Bao1#; Feng Liu2#; Qing Xia2; Qingxia Luan3*; Albandri Bin Ammar4; Nessar Ahmed5; Mark Slevin5,6; Kamela Ali7; Donghui Liu5*

1Department of Infectious Diseases, No. 4 People’s Hospital, Zibo, China

2Department of Medical Images, The Affiliated Hospital of Weifang Medical University, China

3Department of Pediatrics, The Affiliated Hospital of Weifang Medical University, China

4Department of Clinical Nutrition, College of Applied Medical Science, University of Hail, Saudi Arabia

5Department of Life Sciences, Manchester Metropolitan University, UK

6The University of Medicine and Pharmacy of Targu Mures, Romania

7Yanbu College of Applied Medical Sciences, Taibah University, Saudi Arabia

#These authors have contributed equally to this article.

*Corresponding author: Qingxia Luan Department of Pediatrics, The Affiliated Hospital of Weifang Medical University, Shandong, 261031, China. Donghui Liu, Department of Life Sciences, Manchester Metropolitan University, Manchester, M1 5GD, UK Tel: +44 13505361251 Email: [email protected]

Received: September 22, 2023 Accepted: October 13, 2023 Published: October 20, 2023


Impaired wound healing is associated with hyperglycaemia in patients with diabetes. Hyperglycaemia induces protein glycation and the formation of Advanced Glycation End-Products (AGEs). The accumulation of AGEs in the body results in the structural and functional modification of tissue proteins. This study was conducted to evaluate compounds with antiglycation activities (S-Ally1 Cysteine (SAC), N-Acetylcysteine (NAC) and the mimic compound A). The extent of glycation in the presence and absence of several inhibitors was assessed via several methods including fluorescence, Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)-silver stain, Western blotting, and Enzyme-Linked Immunosorbent Assays (ELISA). Additionally, this research aimed to evaluate and quantify the potential of Human Adipose Mesenchymal Stem Cells (hADMSCs) to uptake and release these drugs as potential therapeutics. To achieve this, hADMSCs were primed with a combination of SAC/NAC and mimic compound A and their concentrations were analysed using High-Performance Liquid Chromatography (HPLC). The SAC/NAC and mimic compound A prohibit the formation of AGEs while the Conditioned Medium (CM) from SAC/NAC- and compound A-loaded hADMSCs induced cell migration and tube formation in BAECs. hADMSCs provide a unique opportunity for the development of an innovative targeting and drug-delivery system which could effectively deliver therapeutics to specific regions of wounds or other damaged tissues. The data provided demonstrate the potential of hADMSCs as a drug delivery method with the potential to improve wound healing, and it may offer potential therapeutic targeting for the development of diabetic complications.

Keywords: Diabetes; SAC/NAC; Mimic compound A; Wound healing


The presence of Advanced Glycation End-Products (AGEs) in the wounds of diabetic patients results in continued chronic inflammation which can prevent the wound from progressing to the next stage of healing (matrix deposition and remodelling) and thereby prevents healing from taking place [1].

Many pathological factors affect tissue repair in diabetes mellitus [2] such as atherosclerosis, development of major vascular stenosis or occlusion, and renal failure. Atherosclerosis can lead to an embolism in proximal vessels which results in decreased blood flow, insufficient oxygen delivery and cancause damage to the toes. Diabetic people are more prone to the development of neuropathy and infectious diseases due to immunosuppression. Moreover, in diabetes and venous stasis disease, oedema impairs wound healing.

The organosulphur compounds can be separated into water-soluble and oil-soluble constituents. Water-soluble organosulphur components include SAC (S-allyl cysteine) (Figure-1) and NAC (N-acetylcysteine) (Figure 1) [3]. SAC is a major compound which is primarily observed in garlic extracts from alcoholic and aqueous bases [4]. NAC has a –SH residue donor and a nucleophile [5], deriving from gamma-glutamyl-S-allyl-L-cysteine. A typical antidiabetic treatment combined with garlic has demonstrated the ability to improve antihyperlipidemic activity and glycaemic control indicating that garlic could be a beneficial and suitable addition for patients managing hyperlipidaemia and diabetes [6].