Selective Destruction of Capillaries in Pancreatic Islets Induced by Diabetogenic Zinc Binding Chemicals as Possible Cause of Aggravation of Diabetes

Short Communication

Austin Pancreat Disord. 2017; 1(2): 1009.

Selective Destruction of Capillaries in Pancreatic Islets Induced by Diabetogenic Zinc Binding Chemicals as Possible Cause of Aggravation of Diabetes

Meyramov GG¹*, Kartbaeva GT¹, Shaybek AS¹, Laryushina EM², Dupont ON¹, Alina AR¹, Andreewa AP¹ and Abraimova AG²

¹Buketov Karaganda State University, Kazakhstan

²Karaganda State Medical University, Kazakhstan

*Corresponding author: Meyramov GG, Buketov Karaganda State University, Kazakhstan

Received: August 16, 2017; Accepted: August 28, 2017; Published: September 04, 2017

Background

Administration of Diabetogenic Zinc-Binding Chelat Active Chemicals (DZC) as Dithizon (DZ) and more that 10 derivatives of 8-Oxyquinolin (8OXY) forming in B-cells of pancreas (rabbits, mice, cats, dogs, hamsters, human embryo) of toxic complexes “Zn-chelator” which result destruction of 90-100% of B-cells within 15-30 min [1-5]. It was established that diabetes caused by DZC accompanied by fragmental destruction in islets of capillaries wall and developing of disturbances of blood circulation in islets [6]. Previously these changes were explained as vascular changes typical for diabetes and developed as late vascular complication not as result of local direct influence of “Zn-chelator” complex on wall.

Aim of Work

To study a possible role formation of “Zn-chelator” complexes in B-cells contacted with wall of capillaries in the pathogenesis of alteration of capillaries.

Methods

Various dosed of water-ammonium solution of Dithizon were injected to 14 rabbits: group 1 (4 animals) - 48, 8-51, 9mg/kg, a maximal dose result binding of all amount of Zn in B-cells and developing of diabetes in all animals; group 2 (6 animals) - 35-40 mg/ kg: maximal binding of Zn-ions in B-cells contacted capillaries wall and partial binding in other B-cells; diabetes developed in majority of animals; group 3 (6 animals) - 12-16 mg/kg: binding of Zn-ions in B-cells located around capillaries only. 2 animals of group 1 groups and 3 from groups 2 and 3 were killed 10-15 min past injection of DZ; frozen sections of pancreas tissue animals were investigated using microscopy with dark condenser and other animals were killed 10-11 days past injection of DZ (groups 1 and 2) and at 17-18th day (group 3). Blood Glucose Control (BG) - before and at 3rd, 6th and 9-10th days; sections of fixed pancreas tissue after staining by aldehyde-fuchsine were investigated [7,8].

Results

Group 1: a large amount of granule of complex “Zn-Dithizon” in cytoplasm of B-cells (Figure1a). Concentration of “Zn-Dithizon” complex concentrated in B-cells contacted capillarie’s wall (K1) (Figure 1b) in compared with photometry [9] of other B-cells (K2): K2/K1 =0, 78±0, 18; BG level 4, 9mM, 10, 7mM, 13, 4m Mand 18, 8mM. Group 2: maximal concentration of “Zn-Dithizon” complex concentrated in B-cells contacted capillaries wall (K1) (Figure 1b) in compared with photometry of other B-cells (K2): K2/K1 =0, 34±0, 04; diabetes was developed in all animals; BG level: 5, 2±0, 5mM, 11, 6±1, 1mM, 10, 8±1, 4mM and 17, 2±2, 6mM fragmental destruction and death of endothelium; microcirculation: blood hyperemia and stasis, exocytosis of erythrocytes.

Citation: Meyramov GG, Kartbaeva GT, Shaybek AS, Laryushina EM, Dupont ON, Alina AR, et al. Selective Destruction of Capillaries in Pancreatic Islets Induced by Diabetogenic Zinc Binding Chemicals as Possible Cause of Aggravation of Diabetes. Austin Pancreat Disord. 2017; 1(2): 1009.