Prohibitins in Reproduction- A Timeline

Review Article

Austin J Reprod Med Infertil. 2016; 3(2): 1042.

Prohibitins in Reproduction- A Timeline

Chowdhury I¹*, Thomas K² and Thompson WE1,3

¹Department of Obstetrics and Gynecology, Morehouse School of Medicine, USA

²Department of Neurobiology, Morehouse School of Medicine, USA

³Department of Physiology, Morehouse School of Medicine, USA

*Corresponding author: Indrajit Chowdhury, Department of Obstetrics and Gynecology, Reproductive Science Research Program, Morehouse School of Medicine, 720 Westview Drive Southwest, Atlanta, GA 30310, USA

Received: September 02, 2016; Accepted: October 15, 2016; Published: October 18, 2016

Abstract

Prohibitins are ubiquitous and an evolutionary conserved protein family that is present in multiple cellular organelles including mitochondria in addition to the nucleus. The Prohibitins are involved in multiple cellular functions such as cellular differentiation, anti-proliferation, morphogenesis and play a major role in maintaining the functional integrity of the mitochondria. Our laboratory and other groups have performed experimental studies on the expression and distribution pattern of prohibitins in various reproductive tissues of different species, which are include mice, rats, pigs, humans and few lower vertebrates and invertebrates. Moreover, recent studies have shown that prohibitins are strongly associated with spermatogenesis, folliculogenesis and the functions of the accessory reproductive organs. In this brief review, we highlight experimental evidence that supports the conserved roles that the prohibitins play in reproductive physiology.

Keywords: Prohibitin (PHB); Repressor of estrogen activity (REA); Reproduction

Introduction

Sexual reproduction is a complex multistep hormonal dependent process where a male gamete, the spermatozoa, fertilizes a female gamete, the ova to form a zygote. In vertebrates, the formation of a mature ova and a sperm are through the process of ovarian folliculogenesis and spermetogenesis respectively under the control of endocrine factors including gonadotropins (follicle stimulating hormone, FSH and luteinizing hormone, LH). During these processes, multiple autocrine and paracrine factors, and steroid hormones play important roles as regulators of folliculogenesis and spermatogensis. The coordinated biosynthesis of steroids in the ovary and the testis are critical for progression of the reproductive cycle, successful ovulation and release of spermatozoa, and eventually fertilization and pregnancy. The binding of gonadotropins to specific membrane G-protein coupled receptors (GPCRs), leads to the activation of multiple signal transduction pathways, including the adenylate cyclase-/cAMP-dependent protein kinase A (PKA) pathway, mitogen-activated protein kinase (MAP kinase) signaling and calcium-/calmodul independent pathways that are known to be involved in the regulation of steroidogenesis and gametogenesis in vertebrates. Furthermore, multiple cross-talk among these signal transduction systems has been well documented. Interestingly, several other proteins are involved in the process of gametogenesis and interacting regulatory pathways.

Prohibitins are ubiquitous and evolutionary conserved protein family that belongs to the SPFH family which is characterized by the presence of the stomatin/prohibitin/flotillin/HflK/C (SPFH) domain (also known as the PHB domain). Members of this protein family include prohibitin (PHB/PHB1), repressor of estrogen activity (REA/PHB2), stomatins, plasma membrane proteins of Escherichia coli (HflKC), flotillins, the human insulin receptor (HIR) proteins, the stomatin-like-proteins (SLPs), podocin and the erlins and plant defense proteins [1,2]. Based on extensive database analysis approximately 1800 PHB domain-containing proteins exist which includes 340 animal proteins and 142 mammalian proteins that are present across all organisms. Currently our understanding of the complex biology of the prohibitins in reproductive physiology is limited. However, our laboratory and other groups have performed a number of experimental studies on the expression and distribution pattern of PHB and REA in various reproductive tissues of different species. These studies are performed in mice, rats, pigs, humans and few lower vertebrates and invertebrates including the red crayfish Cherax quadricarinatus, Octopus tankahkeei, Chinese mitten crab Eriocheir sinensis, Danio rerio, Gallus gallus, Salmo salar, and Bos taurus. Moreover, recent studies have shown that prohibitins are strongly associated with spermatogenesis, folliculogenesis and functions of the accessory reproductive organ. In this brief review, we highlight some of the experimental evidence supporting a conserved role for the prohibitins in reproductive physiology.

Prohibitins

In humans, the PHB gene (hPHB) spans ~11 kb with 7 exons and map to chromosome locus 17q21 [3]. The first exon and a small portion of the second exon comprise the 5’ untranslated region, whereas the largest exon, exon 7 contains ~700 bp of 3’ untranslated RNA. Several transcripts of the PHB gene are transcribed with varying lengths of 3’ untranslated region [4]. The longer transcripts are present at higher levels in proliferating tissues and cells [5]. The abundance of PHB mRNA is inversely related to markers of cellular proliferation in different cells and tissues [6-10]. Comparative genomic alignment studies have shown that the human and rat PHB genes are similar except for intron 2 and 3, which are ~1 kb larger in the rat gene [11]. The hPHB gene encodes ~30 kDa protein, also known as B-cell receptor associated protein-32 (BAP32) gene. PHB contains four highly conserved domains, namely, an N-terminal hydrophobic domain; a PHB domain (amino acid residues 26-187) encoded by exon 3, 4 and 5, and which is conserved from protozoa to mammals; a coiled-coil (CC) alpha helices domain (amino acids residues 177–211) present at the C-terminal end of the protein, and encoded largely by exon 6; and a putative nuclear export sequence (amino acid residues 257 to 270) which present at the C-terminal. The N-terminal hydrophobic domain is critical for its attachment to the mitochondrial inner membrane, whereas the CC-domain is important for protein–protein interactions.

The human PHB2 (REA/hPHB2) [12], also referred as prohibitone [13]/B-cell receptor associated protein-37 (BAP37) [14] gene (PHB2) is located at chromosome 12p13 [15]. This gene has 10 exons, with smaller introns than PHB and spans ~5.3 kb. The REA/PHB2/BAP37 gene encodes a protein of ~37 kDa. Similar to PHB structure, REA has a PHB domain, which is encoded by residues 39–201; a CCdomain present at the C terminal end of the protein; a putative signal peptide (residues 1–36), and a putative nuclear localization signal peptide (residues 86–89). In eukaryotes PHB and PHB2 have highly conserved PHB domains. The PHB protein is 54% homologous with PHB2 [16,17] and has a single amino acid difference between rodents and humans [11]. Orthologues of the PHB gene have been identified in several organisms including bacteria [18,19], plants [20,21], Trypanosoma brucei [22], Saccharomyces cerevisiae (yeast) [23,24], Caenorhabditis elegans [25] and Drosophila [26].

Prohibitins and Reproduction

Distinct differences in PHB and PHB2 (REA) levels have been observed during ovarian folliculogenesis, spermatogenesis, in mammary gland development, and in uterine tissue. Immunolocalization, Western blotting and immunogold staining of ovaries and testis had shown that both PHB and PHB2 (REA) are present in multiple cellular locations including mitochondria and nucleus, suggesting that they play additional roles in those cellular compartments. Due to complex post-translational modification and changes in cellular localization occurring during different physiological states, it has been difficult to identify the distinct roles that these prohibitins isoforms play in reproductive organs. Currently ours and other research groups are trying to understand the functional roles that the prohibitins play in reproduction by utilizing conditional knock-out mice. We have highlighted a few important findings on the functional roles that PHB and REA play in reproductive physiology in Table 1 and 2.

Table 1: Prohibitins role in female reproduction.





  

    
Year and References 

    
Critical findings 

  

  

    
[27]

    
    
      
  • Identified and characterized prohibitin (PHB) as an    intracellular protein (p28 kDa) during differentiation of rat granulosa cells    (GCs) isolated from preantral and early antral follicles. 
    • 
    

  

  

    
[28]

    
    
      
  • PHB was localized within rat GCs of infant and    juvenile ovaries. 
    • 
      
  • A strong expression pattern of PHB in rat oocyte at all    stages of follicular development, in rat corpus luteum and in follicles    undergoing atresia.
    • 
    

  

  

    
[29]

    
    
      
  • PHB roles were confirmed in mitochondrial structure and    function during growth and differentiation of GCs. 
    • 
    

  

  

    
[30]

    
    
      
  • PHB gene expression profiles were analyzed in normal and in    carcinoma-induced female rat mammary gland. 
    • 
    

  

  

    
[25] 

    
    
      
  • Demonstrated the roles of    prohibitin (PHB and REA) complex for Caenorhabditiselegans (C. elegans) embryonic development,    and necessary for normal mitochondrial morphology and respiration. 
    • 
    

  

  

    
[31]

    
    
      
  • GnRH dependent PHB expression is important for maturation    of T lymphocytes and rat thymic growth. 
    • 
    

  

  

    
[32]

    
    
      
  • PHB was immunolocalized in rat GCs, theca-interstitial    cells, and the oocyte; and in porcine oocytes, zygotes, and blastocyst.
    • 
      
  • Gonadotropin dependent PHB expression was inversely    correlated to PCNA expression during follicular maturation and positively    co-localized with P450scc.
    • 
      
  • In atretic follicles, germinal vesicle-stage oocytes,    zygotes, and blastocysts PHB was translocated from the cytoplasm to the nucleus. 
    • 
    

  

  

    
[33] 

    
    
      
  • In human fundus, the expressions of repressor of estrogen    receptor activity (REA) is reduced during labor, and suggest that REA gene is    involved in regulatory pathways of estrogen receptor alpha (ERA) activity.
    • 
    

  

  

    
[34]

    
    
      
  • REA is required for the maintenance of estrogen receptor    (ER) activity and normal mammary gland development. The reduction or loss of    REA function promotes over-activation of ER and increase breast cancer risk    in humans. 
    • 
    

  

  

    
[35-37]

    
    
      
  • In rat GCs, mitochondrial PHB act through phospho-PHB    (pPHB)-MEK-pERK pathway and regulates the Bcl/Bcl-xL which inhibit Bax-Bak    expression. These events directly inhibit the release of cytochrome c from    the inter-mitochondrial space and inhibit downstream activation of cleaved    caspase 3. 
    • 
    

  

  

    
[38]

    
    
      
  • In rainbow trout (Oncorhynchusmykiss),    a higher REA mRNA abundance in eggs inhibits development of embryo. 
    • 
    

  

  

    
[39]

    
    
      
  • Demonstrated a    novel mechanism for control of estrogen-induced luminal epithelial    proliferation involving uterine endometrial stromal (ST) Kr�ppel-like factor 9 (KLF9)    regulations of paracrine factor(s) to repress epithelial expression of    co-repressor REA/PHB2 in mice. 
    • 
    

  

  

    
[40]

    
    
      
  • PHB was found to be an    estrogen-regulated gene essential for uterine development and function in mice, and    selectively required for estrogen-regulated gene expression in uterus. 
    • 
    

  

  

    
[41]

    
    
      
  • Studies demonstrated that REA is essential for mammary    gland development and has a gene dosage-dependent role in the regulation of    stage-specific physiological functions of the mammary gland during pregnancy    and lactation stages. 
    • 
    

  

  

    
[42]

    
    
      
  • In cloned placenta of domestic    cat (Feliscatus), the expression of    PHB and cathepsin D (CD) were correlated with the generation of reactive    oxygen species (ROS), leading to decreased mitochondrial membrane potential    and telomeric DNA, which are associated with cellular senescence and    apoptosis.  The abnormal PHB protein patterns were associated with    impaired development, and hence decreased fetal viability. 
    • 
    

  

  

    
[43]

    
    
      
  • REA expression was intense in both the perinuclear    cytoplasm and the nucleus in GCs from follicles at all stages of development    in bovine. However, REA expression was less intense in thecal tissue. 
    • 
    

  

  

    
[44,45]

    
    
      
  • PHB was regulated by follicle    stimulating hormone (FSH) in a follicular stage-dependent manner in vitro in pre-antral GCs isolated    from diethylstilbestrol (DES) treated rats and antral GCs isolated from    equine chorionic gonadotropin (eCG)-treated rats. 
    • 
    

  

  

    
[46]

    
    
      
  • REA physiologically restrained    human endometrial stromal cell decidualization, controlling the timing and    magnitude of decidualization to enable proper coordination of uterine    differentiation with concurrent embryo development that is essential for    implantation and optimal fertility. 
    • 
    

  

  

    
[47]

    
    
      
  • The aberrant expression of glycolysis-related enzymes in    human endometrioma tissue was associated with enhanced glycolytic metabolism.    The malignant-like feature may be partially caused by low-expression of PHB    gene in endometriotic stromal cells. 
    • 
    

  

  

    
[48]

    
    
      
  • In humans, REA modulated crosstalk among multiple cell    types in the uterine tissue and host background, serving as a brake on the    estradiol-ER axis and restraining multiple aspects that contribute to the    pathologic progression of endometriosis. 
    • 
    

  

  

    
[49]

    
    
      
  • Folicle stimulating hormone    (FSH)-dependent PHB/pPHB upregulation in GCs is required to sustain the    differentiated state of GCs. 
    • 
    

  










Table 1: Prohibitins role in female reproduction.

Table 2: Prohibitins role in male reproduction.





  

    
Year and References 

    
Critical findings 

  

  

    
 [5]

    
    
      
  • Identified and characterized prohibitin (PHB) as an    intracellular protein (p30 kDa) in rat seminiferous epithelium. 
    • 
      
  • PHB protein was    constitutively present in adult Leydig cells and Sertoli cells at all    stages.PHB protein were very low in preleptotene spermatocytes, very high in    leptotene spermatocytes, and very low in zygotene spermatocytes. 
    • 
      
  • In pachytene spermatocytes, PHB was very high in stages    VII-XI and was minimal during stages XII and XIV. 
    • 
      
  • No PHB protein was detected in spermatogonia and    spermatocytes. 
    • 
    

  

  

    
 [50]

    
    
      
  • PHB was identified as one of the ubiquitinated    substrates that makes the sperm mitochondria recognizable by the egg's    ubiquitin-proteasome dependent proteolytic machinery after fertilization and    most likely facilitates the marking of defective spermatozoa in the    epididymis for degradation. 
    • 
    

  

  

    
 [51]

    
    
      
  • PHB protein was identified and    characterized in cephalopod Octopus    tankahkeei (O. tankahkeei)    during spermiogenesis.
    • 
    

  

  

    
 [52]

    
    
      
  • PHB cDNA was cloned and    sequenced from testes of the crab Eriocheirsinensis.
    • 
    

  

  

    
[53]

    
    
      
  • The PHB gene and its protein    were characterized in crayfish (Cherazquadricarinatus)    during spermatogenesis and PHB protein was localized in the innermitochondrial    membranes of sperm 
    • 
      
  • The strongest PHB signal was found in spermatogonia, and    lower levels in secondary spermatocytes, and weak or absent expression in    mature sperm.
    • 
    

  

  

    
 [54]

    
    
      
  • Demonstrated a role for PHB in    GnRH-induced cell death in mature gonadotropes, which is crucial for the    normal development and function of the reproductive axis. 
    • 
    

  

  

    
 [55]

    
    
      
  • PHB was co-localized in mitochondria from the late stage of    spermiogenesis of macrura crustacean species, P. clarkia. 
    • 
    

  

  

    
 [56]

    
    
      
  • PHB gene was cloned and characterized in the Chinese    fire-bellied newt Cynopsorientalis,    and expressed during spermatogenesis and spermiogenesis.
    • 
      
  • PHB existed during the whole period    of spermatogenesis and spermiogenesis. In the mature germ cells, the signals    of prohibitins were weak and aggregated at the end of the cell, where as in    the sertoli cells had abundant PHB concentrations. 
    • 
    

  










Table 2: Prohibitins role in male reproduction.

Conclusion

At present, we are just beginning to understand the critical roles that PHB and PHB2 (REA) play in ovarian, uterine, mammary gland, testis and accessory reproductive organ functions. Although current knowledge indicates that PHB and REA are involved in regulating the fate of folliculogenesis, spermatogenesis and other reproductive processes. A number of basic questions still remain to be answered. These include physiological role that PHB and PHB2 (REA) play in regulating gene expression and signaling in spermatogenesis, folliculogenesis, and the functions of the accessory reproductive organs.

Acknowledgement

This study was supported in part by National Institutes of Health Grants 1SC3GM113751, 1RO1HD057235 and G12-RR03034. This investigation was conducted in a facility constructed with support from Research Facilities Improvement Grant #C06 RR18386 from NIH/NCRR.

References

  1. Nadimpalli R, Yalpani N, Johal GS, Simmons, CR. Prohibitins, stomatins and plant disease response genes compose a super family that controls cell proliferation, ion channels regulation and death. J Biol Chem. 2000; 275: 29579-29586.
  2. Nijtmans LG, Artal SM, Grivell LA, Coates PJ. The mitochondrial PHB complex: roles in mitochondrial respiratory complex assembly, aging and degenerative disease. Cell Mol Life Sci. 2002; 59: 143-155.
  3. Sato T, Saito H, Swensen J, Olifant A, Wood C, Danner D, et al. The human prohibitin gene located on chromosome 17q21 is mutated in sporadic breast cancer. Cancer Res. 1992: 52: 1643-1646.
  4. Jupe ER, Liu XT, Kiehlbauch JL, McClung JK, Dell’Orco RT. The 3’ untranslated region of prohibitin and cellular immortalization. Exp Cell Res.1996; 224: 128-135.
  5. Choongkittaworn NM, Kim KH, Danner DB, Griswold MD. Expression of prohibitin in rat seminiferous epithelium. Biol Reprod.1993; 49: 300-310.
  6. Hussain-Hakimjee EA, Peng X, Mehta RR, Mehta RG. Growth inhibition of carcinogen-transformed MCF-12F breast epithelial cells and hormone-sensitive BT-474 breast cancer cells by 1alpha-hydroxyvitamin D5. Carcinogenesis. 2006; 27: 551-559.
  7. Jupe ER, Liu XT, Kiehlbauch JL, McClung JK, Dell'Orco RT. Prohibitin antiproliferative activity and lack of heterozygosity in immortalized cell lines. Exp Cell Res.1995; 218: 577-580.
  8. Lumpkin CKJr, Moore TL, Tarpley MD, Taylor JM, Badger TM, McClung JK. Acute ethanol and selected growth suppressor transcripts in regenerating rat liver. Alcohol. 1995; 12: 357-362.
  9. Miyamoto S, Qin J, Safer B. Detection of early gene expression changes during activation of human primary lymphocytes by in vitro synthesis of proteins from polysome associated mRNAs. Protein Sci. 2001; 10: 423-433.
  10. Tanno S, Fukuda I, Saito Y, Ogawa K. Prohibitin expression is decreased in the regenerating liver but not in chemically induced hepatic tumors in rats. Jpn J Cancer Res.1997; 88: 1155-1164.
  11. Altus MS, Wood CM, Stewart DA, Roskams AJ, Friedman V, Henderson T, et al. Regions of evolutionary conservation between the rat and human prohibitin-encoding genes. Gene. 1995; 158: 291-294.
  12. Montano MM, Ekena K, Delage-Mourroux R, Chang W, Martini P, Katzenellenbogen BS. An estrogen receptor-selective coregulator that potentiates the effectiveness of antiestrogens and represses the activity of estrogens. Proc Natl Acad Sci USA.1999; 96: 6947-6952.
  13. Lamers MC, Bacher S. Prohibitin and prohibitone, ubiquitous and abundant proteins that are reluctant to reveal their real identity. Int Arch Allergy Immunol. 1997; 113: 146-149.
  14. Terashima M, Kim KM, Adachi T, Nielsen PJ, Reth M, Kohler G, et al. The IgM antigen receptor of B lymphocytes is associated with prohibitin and a prohibitin related protein. EMBO J. 1994; 13: 3782-3792.
  15. Ansari-Lari MA, Shen Y, Muzny DM, Lee W, Gibbs RA. Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination. Genome Res.1997; 7: 268-280.
  16. Chowdhury I, Garcia-Barrio M, Harp D, Thomas K, Matthews R, Thompson WE. The emerging roles of prohibitins in folliculogenesis. Front. Biosci. (Elite Ed). 2012; 4: 690-699.
  17. Gamble SC, Chotai D, Odontiadis M, Dart DA, Brooke GN, Powell SM, et al. Prohibitin, a protein downregulated by androgens, represses androgen receptor activity. Oncogene. 2007; 26: 1757-1768.
  18. de Monbrison F, Picot S. Introducing antisense oligonucleotides into Pneumocystis carinii. J Microbiol Methods. 2002; 50: 211-213.
  19. Narasimhan S, Armstrong M, McClung JK, Richards FF, Spicer EK. Prohibitin, a putative negative control element present in Pneumocystis carinii. Infect Immun.1997; 65: 5125-5130.
  20. Snedden WA, Fromm H. Characterization of the plant homologue of prohibitin, a gene associated with antiproliferative activity in mammalian cells. Plant Mol Biol. 1997; 33: 753-756.
  21. Takahashi A, Kawasaki T, Wong HL, Suharsono U, Hirano H, Shimamoto K. Hyperphosphorylation of a mitochondrial protein, prohibitin, is induced by calyculin A in a rice lesion-mimic mutant cdr1. Plant Physiol. 2003; 132: 1861-1869.
  22. Welburn SC, Murphy NB. Prohibitin and RACK homologues are up-regulated in trypanosomes induced to undergo apoptosis and in naturally occurring terminally differentiated forms. Cell Death Differ.1998; 5: 615-622.
  23. Kirchman PA, Miceli MV, West RL, Jiang JC, Kim S, Jazwinski SM. Prohibitins and Ras2 protein cooperate in the maintenance of mitochondrial function during yeast aging. Acta Biochim Pol. 2003; 50: 1039-1056.
  24. Tatsuta T, Model K, Langer T. Formation of membrane bound ring complexes by prohibitin in mitochondria. Mol Biol Cell. 2005; 16: 248-259.
  25. Artal-Sanz M, Tsang WY, Willems EM, Grivell LA, Lemire BD, van der Spek H, et al. The mitochondrial prohibitin complex is essential for embryonic viability and germline function in Caenorhabditis elegans. J Biol Chem 2003; 278: 32091-32099.
  26. Eveleth DD Jr, Marsh JL. Sequence and expression of the Cc gene, a member of the dopa decarboxylase gene cluster of Drosophila: possible translational regulation. Nucleic Acid Res. 1986; 14: 6169-6183.
  27. Thompson WE, Sanbuissho A, Lee GY, Anderson E. Steroidogenic acute regulatory (StAR) protein (p25) and prohibitin (p28) from cultured rat ovarian granulosa cells. J. Reprod Fertil. 1997; 109: 337-348.
  28. Thompson WE, Powell JM, Whittaker JA, Sridaran R, Thomas KH. Immunolocalization and expression of prohibitin, a mitochondrial associated protein within the rat ovaries. Anat Rec. 1999; 256: 40-48.
  29. Thompson WE, Branch A, Whittaker JA, Lyn D, Zilberstein M, Mayo KE, et al. Characterization of prohibitin in a newly established rat ovarian granulose cell line. Endocrinology.2001; 142: 4076-4085.
  30. Shan L, He M, Yu M, Qiu C, Lee NH, Liu ET, et al. cDNA microarray profiling of rat mammary gland carcinomas induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and 7,12-dimethylbenz[a]anthracene. Carcinogenesis. 2002; 23: 1561-1568.
  31. Dixit VD, Sridaran R, Edmonsond MA, Taub D, Thompson WE. Gonadotropin releasing hormone attenuates pregnancy-associated thymic involution and modulates the expression of antiproliferative gene product prohibitin. Endocrinology. 2003; 144: 1496-1505.
  32. Thompson WE, Asselin E, Branch A, Stiles JK, Sutovsky P, Lai L, et al. Regulation of prohibitin expression during follicular development and atresia in the mammalian ovary. Biol Reprod. 2004; 71: 282-290.
  33. Bukowski R, Hankins GD, Saade GR, Anderson GD, Thornton S. Labor-associated gene expression in the human uterine fundus, lower segment, and cervix. PLoS Med. 2006; 3: e169.
  34. Mussi P, Liao L, Park SE, Ciana P, Maggi A, Katzenellenbogen BS, et al. Haploinsufficiency of the corepressor of estrogen receptor activity (REA) enhances estrogen receptor function in the mammary gland. Proc Natl Acad Sci USA. 2006; 103: 16716-16721.
  35. Chowdhury I, Xu W, Stiles JK, Zeleznik A, Yao X, Matthews R, et al. Apoptosis of rat granulosa cells after staurosporine and serum withdrawal is suppressed by adenovirus-directed over expression of prohibitin. Endocrinology. 2007; 148: 206-217.
  36. Chowdhury I, Branch A, Olatinwo M, Thomas K, Matthews R, Thompson WE. Prohibitin (PHB) acts as a potent survival factor against ceramide induced apoptosis in rat granulosa cells. Life Sci. 2011; 89: 295-303.
  37. Chowdhury I, Thompson WE, Welch C, Thomas K, Matthews R. Prohibitin (PHB) inhibits apoptosis in rat granulosa cells (GCs) through the extracellular signal-regulated kinase 1/2 (ERK1/2) and the Bcl family of proteins. Apoptosis. 2013; 18: 1513-1525.
  38. Bonnet E, Fostier A, Bobe J. Microarray-based analysis of fish egg quality after natural or controlled ovulation. BMC Genomics. 2007; 8: 55-71.
  39. Pabona JM, Velarde MC, Zeng Z, Simmen FA, Simmen RC. Nuclear receptor co-regulator Krüppel-like factor 9 and prohibitin 2 expression in estrogen-induced epithelial cell proliferation in the mouse uterus. J Endocrinol. 2009; 200: 63-73.
  40. He B, Kim TH, Kommagani R, Feng Q, Lanz RB, Jeong JW, et al. Estrogen-regulated prohibitin is required for mouse uterine development and adult function. Endocrinology. 2011; 152: 1047-1056.
  41. Park S, Zhao Y, Yoon S, Xu J, Liao L, Lydon J, et al. Repressor of estrogen receptor activity (REA) is essential for mammary gland morphogenesis and functional activities: studies in conditional knockout mice. Endocrinology. 2011; 152: 4336-4349.
  42. Bang JI, Bae DW, LeeHS, Deb GK, Kim MO, Sohn SH, et al. Proteomic analysis of placentas from cloned cat embryos identifies a set of differentially expressed proteins related to oxidative damage, senescence and apoptosis. Proteomics. 2011; 11: 4454-4467.
  43. Salvetti NR, Alfaro NS, Velázquez MM, Amweg AN, Matiller V, Díaz PU, et al. Alteration in localization of steroid hormone receptors and coregulatory proteins in follicles from cows with induced ovarian follicular cysts. Reproduction. 2012; 144: 723-735.
  44. Wang Q, Leader A, Tsang BK. Inhibitory roles of prohibitin and chemerin in FSH-induced rat granulosa cell steroidogenesis. Endocrinology. 2013; 154: 956-967.
  45. Wang Q, Leader A, Tsang BK. Follicular stage-dependent regulation of apoptosis and steroidogenesis by prohibitin in rat granulosa cells. J Ovarian Res. 2013; 6: 6-23.
  46. Zhao Y, Park S, Bagchi MK, Taylor RN, Katzenellenbogen BS. The coregulator, repressor of estrogen receptor activity (REA), is a crucial regulator of the timing and magnitude of uterine decidualization. Endocrinology. 2013; 154: 1349-1360.
  47. Qi X, Zhang Y, Ji H, Wu X, Wang F, Xie M, et al. Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis. Reprod Biomed Online. 2014; 29: 761-770.
  48. Zhao Y, Chen Y, Kuang Y, Bagchi MK, Taylor RN, Katzenellenbogen JA, et al. Multiple Beneficial Roles of Repressor of Estrogen Receptor Activity (REA) in Suppressing the Progression of Endometriosis. Endocrinology. 2015; 157: 900-912.
  49. Chowdhury I, Thomas K, Zeleznik A, Thompson WE. Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation. J Mol Endocrinol. 2016; 56: 325-336.
  50. Thompson WE, Ramalho-Santos J, Sutovsky P. Ubiquitination of prohibitin in mammalian sperm mitochondria: possible roles in the regulation of mitochondrial inheritance and sperm quality control. Biol Reprod. 2003; 69: 254-260.
  51. Mao HT, Wang DH, Lan Z, Zhou H, Yang WX. Gene expression profiles of prohibitin in testes of Octopus tankahkeei (ot-phb) revealing its possible role during spermiogenesis. Mol Biol Rep. 2012; 39: 5519-5528.
  52. Mao H, Wang DH, Zhou H, Yang WX. Characterization and expression analysis of prohibitin in the testis of Chinese mitten crab Eriocheirsinensis. Mol Biol Rep. 2012; 39: 7031-7039.
  53. Fang DA, Wang Y, Wang J, Liu LH, Wang Q. Characterization of Cheraxquadricarinatus prohibitin and its potential role in spermatogenesis. Gene. 2013; 519: 318-325.
  54. Savulescu D, Feng J, Ping YS, Mai O, Boehm U, He B, et al. Gonadotropin-releasing hormone-regulated prohibitin mediates apoptosis of the gonadotrope cells. Mol Endocrinol. 2013; 27: 1856-1870.
  55. Dong WL, Hou CC, Yang WX. Mitochondrial prohibitin and its ubiquitination during crayfish Procambarusclarkiispermiogenesis. Cell Tissue Res. 2015; 359: 679-692.
  56. Jin JM, Hou CC, TanFQ, Yang WX. The potential function of prohibitin during spermatogenesis in Chinese fire-bellied newt Cynopsorientalis. Cell Tissue Res. 2016; 363: 805-22.

Citation: Chowdhury I, Thomas K and Thompson WE. Prohibitins in Reproduction- A Timeline. Austin J Reprod Med Infertil. 2016; 3(2): 1042. ISSN:2471-0393