Research Article
Austin J Nutri Food Sci. 2019; 7(7): 1130.
Sensory Optimization of Completely Grape Juice with Added Yam
Andrade AC1, Rodrigues JF2, Oliveira JPL1*, Natividade MMP1 and Bastos SC1
1Departament of Nutrition, Federal University of Lavras, Brazil
2Departament of Agrarian Science, Federal Institute of Minas Gerais, Brazil
*Corresponding author: Oliveira JPL, Department of Nutrition, Federal University of Lavras, Street Instituto, 440, Vila São Francisco, Lavras – MG, Zip code: 37200- 000, Brazil
Received: September 09, 2019; Accepted: October 16, 2019; Published: October 23, 2019
Abstract
Due to the possibility of adding yams to beverages, it is believed that the yam added to the whole grape juice will make it even more nutritious by raising the content of vitamins, minerals, and mainly antioxidant compounds, besides possibly obtaining a more creamy juice, i.e., with innovative technological characteristics. Therefore, the present study aimed to evaluate the effect of the addition of different yam concentrations on the sensory characteristics of completely grape juice. Juice formulations were evaluated through acceptance tests with 75 consumers. Two analyses were performed to evaluate the impact of the product health benefits information on the consumer acceptance. Based on the results of the sensory analysis, it can be concluded that it is possible to add up to 15% of yam in the completely grape juice without depreciating its sensory quality. This addition of yam to the juice can contribute favorably with the nutritional value of this beverage and hence with its sensory acceptance by the consumer. It was possible to observe that information and health concern by consumers contributed to increasing the product acceptability; however, the sensory properties are decisive to elaborate a new product.
Keywords: Grape juice; Health information; Yam; Beverages
Introduction
The population is now becoming more health conscious, but considering the current fast-paced life, people have been looking for practical, healthy foods, i.e., ready-to-eat or serve. This has led to the popularization of natural fruit juices as a healthier alternative in relation to other beverages and soft drinks with high sugar content [1].
Natural fruit juices are part of the daily diet of people of different age groups and provide a deep relationship with health due to its nutritional content, functional and therapeutic qualities. Its consumption is associated with the reduction of diseases such as cancer, neurodegenerative and cardiovascular diseases [1,2].
Grape is one of the most consumed fruits worldwide due to its sensory properties, commercial availability and reasonable price, besides showing relatively high yield. In this sense, grapes are widely used to obtain several products, including grape juice [3]. Grape juice is one of the primary grape derivatives and can be defined as an unfermented and undiluted beverage, obtained from the edible part of the grape (Vitis ssp.) through appropriate technological processes [4].
Grape juice has becoming relevant because it contains a high amount of bioactive compounds, mainly phenolics, such as anthocyanins, catechins, resveratrol, among others, which have antioxidant properties capable of providing health benefits, including prevention of cardiovascular diseases and cancer [5,6].
Changes in eating habits, besides sensory preferences of consumers, have contributed to emerging a new trend related to beverages prepared with fruits and vegetables. The blend of fruits and vegetables can improve sensory properties such as aroma and flavor, besides adding higher nutritional value to beverages. Therefore, the food industries are seeking to develop new juices with vegetables, such as with added yam [7].
Yam is a tuber with a good source of nutrients, being rich in vitamin C and thiamine, minerals (K, P, Ca, Mg, Fe and Cu), antioxidant fibers and compounds (phytosterols) [8,9]. Thereby, yam has potentially positive health effects and can increase the antioxidant capacity [10], acting as cardioprotective, anti-inflammatory and lipid metabolism, reducing the triglycerides and improving the cholesterol profile [11,12].
Besides providing potential health effects, the addition of yams may contribute with critical technological characteristics in product development due to its high starch content [11]. According to [13] Pelegrini and Carrasqueira (2008), yam juice is a much-valued product. These same authors prepared a vitamin yam juice and another of pineapple, and found that yam juice was the most preferred after sensory analysis [14]. Martins et al., (2011) also obtained good acceptability for a sports drink prepared from the concentrated juice of fruits and vegetables containing yams. This fact suggests that the addition of yams to beverages can improve sensory acceptability and contribute to the product’s nutritional value.
Due to the possibility of adding yams to beverages, it is believed that the yam added to the whole grape juice will make it even more nutritious by raising the content of vitamins, minerals, and mainly antioxidant compounds, besides possibly obtaining a more creamy juice, i.e., with innovative technological characteristics. Therefore, the present study aimed to evaluate the effect of the addition of different yam concentrations on the sensory characteristics of completely grape juice.
Material and Methods
Samples
Completely grape juice (Campino®) and yam (Dioscorea sp.) were used for the product processing. Raw materials were purchased in the Lavras commerce derived from a single batch.
Juice formulations were determined by preliminary tests, being established ratios of 5%, 10%, 15%, 20% and 25% of yams. According to previous assays, a yam addition greater than 25% mischaracterized the completely grape juice concerning taste, color and texture and therefore levels above this were avoided.
Yam was washed in running water in order to remove the larger soils, sanitized in sodium hypochlorite (NaClO) solution at 200 ppm for 30 min and thereafter washed in running water; afterward, it was peeled, cut in cubes and ground in a blender (Walita®) together with whole grape juice [14,15] (Martins et al., 2011; Rodrigues et al., 2015), for approximately 4 min until complete yam dissolution. The prepared beverages were submitted to the physical and chemical analyses and were placed in the refrigerator for approximately 2 h for temperature balance. After this period, the sensory tests were performed.
Sensory analyses
Sensory analyses were performed at the Sensory Analysis Laboratory of the Federal University of Lavras with healthy individuals and without any restriction to the analyzed products. The study was performed according to the Research Ethics Committee on Human Beings of the Federal University of Lavras (CAAE: 34331414.6.0000.5148).
Juice formulations were evaluated through acceptance tests with 75 consumers through SensoMaker software [16] (Pinheiro et al., 2013). Two analyses were performed. In the first analysis, the consumers did not obtain any information about the product (ingredients, benefits, among others). However, in the second test, previously to sensory analysis, a pamphlet was shown to each tester informing the ingredients and benefits of whole grape juice with added yam, such as sugar-free, water, preservatives and colorings, and the presence of fibers, minerals and antioxidants, besides their potential health benefits (prevention of chronic diseases, such as cardiovascular disease, improvement of cholesterol profile, reduction of triglycerides and other diseases associated with oxidative stress).
Four properties were evaluated: appearance, aroma, taste and overall impression. To this end, the tasters were instructed to taste the samples from left to the right and indicate how much they liked or disliked each one, using a structured 9-point hedonic scale, which ranged from “1-dislike extremely” to “9-like extremely” [15,17] (Rodrigues et al., 2015; Minin, 2010), and a structured five-point Likert scale, which ranged from 1 “certainly would not buy” to 5 “buy certainly” for the purchase intention.
In both sensory tests, grape juice samples (30 ml) without and with yam were served to the tasters. They were served in disposable white plastic cups, identified by three-digit numbers arranged in a balanced order, according to Wakeling and McFie [18] (Wakeling and McFie, 1995). Evaluations used white light. The tests were performed in individual cabins, at room temperature, with adequate lighting and without the interference of odors and noise [19] (Bowles and Demiate, 2006).
Statistical analysis
Sensory results were evaluated through analysis of variance and Tukey test, and through internal preference mapping, using Sensomaker software [16] (Pinheiro et al., 2013). Physical and chemical data were expressed as the triplicate average and the respective standard deviation.
Results and Discussion
Sensory analysis
The results of acceptance tests (with and without information on the product benefits) were evaluated by three-way preference mapping (Figure 1A and 1B).
Figure 1: Three-way preference maps obtained for the formulations of grape juice with added yam evaluated without (A) and with prior information (B) regarding the evaluated product.
Based on the preference maps, it is observed that the tasters preferred the samples added with 5, 10 and 15% yam in both evaluations. Moreover, all the evaluated sensory properties (taste, aroma, appearance and overall impression), as well as the purchase intention, contributed to their greater acceptance. Considering this and considering that the properties that constitute the sensory quality are integrated into the brain, as an overall impression quality [20,21] (Oliveira, 2010; Rodrigues et al., 2014), this property was considered to evaluate the best concentrations of added yam, determined according to the preference of tasters.
The acceptance averages for the overall impression obtained among the samples within each of the evaluations and between the evaluations (with and without information on the product benefits are presented in (Table 1). The analysis of variance indicated that there was a significant difference (p≤0.05) among the samples in both evaluations and between the two evaluations.
Samples
Overall impression mean
Evaluation without information about the product benefits
Evaluation with prior information about the product benefits
5% yam
7.07
± 1.65 a A
7.32
± 1.55 a A
10% yam
6.43
± 1.85 b A
6.59
± 1.50 b A
15% yam
6.40
± 1.70 b A
6.85
± 1.61 ab A
20% yam
5.16
± 2.10 c A
5.31
± 1.78 c A
25% yam
4.84
± 2.24 c A
5.81
± 1.83 c B
Equal lowercase letters on the same column indicate that the samples do not differ statistically by Tukey test; Equal capital letters on the same row indicate that the evaluations (with and without information on the product benefits) do not differ statistically among themselves by Tukey test.
Table 1: Acceptance averages for the overall impression obtained among the samples within each of the evaluations and between the evaluations (with and without information on the product benefits).
In (Table 1) is shown that in both evaluations (without and with prior information on the product benefits), the formulation of grape juice added with 5% yam obtained a sensory acceptance greater than the others, with scores between 7-like moderately and 8-like very much, followed by samples with 10 and 15% of yams that did not statistically differ among themselves and obtained scores between 6-like slightly and 7-like moderately, which also characterize good acceptance of these products. Juices with 20 and 25% of yam were the least preferred, with scores between 5-neither like nor dislike and 6-like slightly.
In a similar study, [15] Rodrigues et al., (2015) found that the increased yam concentration in passion fruit juice reduced its acceptance; thus, it was possible to add up to 10% of yam in passion fruit juice without worsening the overall impression. However, the samples most accepted by consumers contained the highest sugar concentrations (6 to 8%), showing that the sweet taste contributed to improving the beverage acceptance.
Therefore, it was verified that the consumers more accepted the whole grape juice with added yam, is possible to add 5% more of yam concerning the passion fruit juice in the study of [15] Rodrigues et al., (2015) without changing the overall impression. Another observed point was that the whole grape juice used in the present study does not show added sugar as passion fruit juice did. Nevertheless, a greater acceptance was obtained, demonstrating that the juice flavor can influence the amount of added yam.
Yam is food with high nutritional value, rich in nutrients, fiber, antioxidant compounds, and starch, favoring its addition in several beverages [8,9] (Xu et al., 2009; Ferraro et al., 2016). to improve both the sensory properties as nutritional aspects. Thus, the beverage acceptance could be promoted by reporting its benefits to the consumers.
Regarding the effect of information on the product’s sensory acceptance, it is observed that the knowledge about the benefits of the yam addition to grape juice positively influenced its acceptance when added at a higher concentration of yam. This was observed for formulation E, with 25% of yam, which obtained a statistically average score (between 5-neither like nor dislike and 6-like slightly) higher to that obtained in sensory analysis without prior information (score between 4-dislike slightly and 5-neither like nor dislike) of the product, taking this formulation to the acceptance region.
According to [22] Fernqvist and Ekelund (2014), information on health benefits in products can be an influential factor in consumers’ choice and sensory perception. In the research performed by [17] Minim (2010), health information increased the acceptance of exotic fruit juices. In the case of [23] Carrilho et al., (2012) the information did not positively influence the sensory characteristics of cookies. Therefore, health information may affect acceptance or not, but the result depends mainly on the product sensory properties. Thereby, the flavor greatly influences the consumer choice.
Therefore, it was possible to observe that information and health concern by consumers contributed to increasing the product acceptability; however, the sensory properties are decisive to elaborate a new product. Taking into consideration the hedonic values obtained in the two sections, the addition of 15% of yam was the best concentration.
Conclusion
Based on the results of the sensory analysis, it can be concluded that it is possible to add up to 15% of yam in the whole grape juice without depreciating its sensory quality. This addition of yam to the juice can contribute favorably with the nutritional value of this beverage and hence with its sensory acceptance by the consumer.
References
- Bhardwaj RL, Nandjal U, Pal A, Jain S. Bioactive compounds and medicinal properties of fruit juice. Fruits. 2014; 69: 391-412.
- Zheng J, Zhou Y, Li S, Zhang P, Zhou T, Xu D, et al. Effects and mechanisms of fruit and vegetable juices on cardiovascular diseases. Int J Mol Sci. 2017; 18: 1-15.
- Granato D, Carrapeiro MM, Fogliano V, Ruth SM. Effects of geographical origin, varietal and farming system on the chemical composition and functional properties of purple grape juices: A review. Trends Food Sci Technol. 2016; 52: 31-48.
- Brasil. Instrução normativa n° 14, de 08 de fevereiro de 2018. Complementação dos padrões de identidade e qualidade do vinho e dos derivados da uva e do vinho. Diário Oficial da União. 2018.
- Nassiri-Asl M, Hosseinzadeh H. Review of the pharmacological effects of Vitis vinifera (Grape) and its bioactive constituents: an update. Phytother Res. 2016; 30: 1392-1403.
- Hasan MM, Bae H. An overview of stress-induced resveratrol synthesis in grapes: perspectives for resveratrol-enriched grape products. Molecules. 2017; 22: 1-18.
- Bhardwaj R, Pandey S. Juice Blends-a way utilization of under-utilized fruits, vegetables, and spices: a review. Crit Rev Food Sci Nutr. 2011; 51: 563-570.
- Xu C, Cai Y, Toyokawa K. Silage preparation and nutritive value of Chinese yam by-product. Animal Sci J. 2009; 80: 398-402.
- Ferraro V, Piccirillo C, Tomlins K, Pintado ME. Cassava (Manihot esculenta Crantz), Yam (Dioscorea spp.) crops, and their derived foodstuffs: safety, security and nutritional value. Crit Rev Food Sci Nutr. 2016; 56: 2714-2727.
- Ajibola CF, Fashakin JB, Fagbemi TN, Aluko RE. Effect of peptide size on antioxidant properties of African yam bean seed (Sphenostylis stenocarpa) protein hydrolysate fractions. Int J Mol Sci. 2001; 12: 6685-6702.
- Chen CT, Wang ZH, Hsu CC, Lin HH, Chen JH. Taiwanese and Japanese yam (Dioscorea spp.) extracts attenuate doxorubicin-induced cardiotoxicity in mice. J Food and Drug Anal. 2017; 25: 872-880.
- Liu Y, Li H, Fan Y, Man S, Liu Z, Gao W, Wang T. Antioxidant and antitumor activities of the extracts from Chinese Yam (Dioscorea opposite Thunb): Flesh and peel and the effective compounds. J Food Sci. 2016; 81: H1553-H1564.
- Pelegrine DHG, Carrasqueira RL. Aproveitamento das proteínas do soro do leite no enriquecimento nutricional de sucos vitaminados. Revista Ciências Exatas e Naturais. 2008; 10: 103-114.
- Martins RC, Chiapetta SC, Paula FD, Gonçalves ECBA. Avaliação da vida de prateleira de bebida isotônica elaborada com suco concentrado de frutas e hortaliças congelado por 30 dias. Alimentação e Nutrição Araraquara. 2011; 22: 623-629.
- Rodrigues JF, Bastos SC, Nunes CA, Pinheiro ACM. Yam addition in passion fruit juice: optimization of the consumer acceptance. Magistra Cruz das Almas. 2015; 27: 169-176.
- Pinheiro ACM, Nunes CA, Vietoris V. SensoMaker: A tool for sensorial characterization of food products. Ciênc Agrotec. 2013; 37: 199-201.
- Minim VPR. Análise Sensorial – Estudo com Consumidores. 2nd edn. Viçosa: Editora da Universidade Federal de Viçosa. 2010.
- Walkeling IN, MacFie JH. Designing consumer trials balanced for first and higher orders of carry-over effect when only a subset of j samples from t may be tested. Food Qual Prefer. 1995; 6: 299-308.
- Bowles S, Demiate IM. Caracterização físico-química de okara em aplicação em pães do tipo francês. Ciên Tecnol Aliment. 2006; 26: 652-659.
- Oliveira, AF. Análise sensorial dos alimentos (Apostila do curso de tecnologia de Alimentos). Universidade Tecnológica Federal do Paraná, Londrina. 2010.
- Rodrigues JF, Junqueira G, Goncalves CS, Carneiro JDS, Pinheiro ACM, Nunes CA. Elaboration of garlic and salt spice with reduced sodium intake. An Acad Bras Ciên. 2014; 86: 2065-2075.
- Fernqvist F, Ekelund L. Credence and the effect on consumer liking of food: A review. Food Qual Prefer. 2014; 32: 340-353.
- Carillo E, Varela P, Fiszman S. Packaging information as a modulator of consumers’ perception of enriched and reduced-calorie biscuits in tasting and non-tasting tests. Food Qual Prefer. 2012; 25: 105-115.